Tehran University of Medical Sciences Journal of Craniomaxillofacial Research 2345-5489 10 2 2023 10 16 46 51 Mohammad Bayat Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. Sarvenaz Karimi Aval Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hosein Amirzade Universal Network of Interdisciplinary Research in Oral and Maxillofacial Surgery (UNIROMS), Universal Scientific Education and Research Network (USERN), Tehran, Iran. Abdolreza Mohamadnia Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Naghmeh Bahrami Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2023 08 08 2023 09 30 Introduction: Loss of tissue and dysfunction of body organs due to injuries and diseases have  caused serious health problems despite the vast advances in Medicien. Bone repair through tissue  engineering is one of the most important areas of attention for specialists in a wide range of departments,  including orthopedics and maxillofacial surgeries.  Materials and Methods: This case-series describes efficient clinical applications of adipose- derived stem cells (AdSCs) in combination with conventional guided bone regeneration as  protected healing space for reconstruction of large alveolar defects after hemi-mandibulectomy.  Results: As the result, this approach represented a considerable amount of three-dimensional  bone formation in presented cases. Regenerating this amount of resected bone tissue rehabilitates  the patients and gives clinicians a sufficient amount of bone and opportunity to insert dental implants  to retrieve patients’ function and aesthetic.  Conclusion: The application of AdSCs can be considered as an efficient treatment for bone regeneration  in large alveolar bone defects.  Keywords: Alveolar bone reconstruction; Mesenchymal stem cell; Adipose-derived stem cells;  Natural bovine bone mineral. https://jcr.tums.ac.ir/index.php/jcr/article/view/452 https://jcr.tums.ac.ir/index.php/jcr/article/download/452/376