Tehran University of Medical Sciences Journal of Craniomaxillofacial Research 2345-5489 3 1 2015 03 15 161 165 EN Shamsolmolok Najafi Department of Oral Medicine and Dental Research Center, Campus International Dentistry Faculty, Tehran University of Medical Sciences, Tehran, Iran. DentalSchool, Tehran University of Medical Sciences. Naghmeh Bahrami Craniomaxillofacial Research center, Tehran University of Medical Sciences, Tehran, Iran. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. Iranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, Iran. Nafiseh Esmaili Autoimmune bulbous diseases research center, Tehran University of Medical Sciences, Tehran, Iran. Mahsa Mohammadzadeh Dentist Narges Gholizade Department of Oral Medicine and Dental Research Center, Dental School, Tehran University of Medical Sciences. Soudabeh Mahdavi Dentist Abdolreza Mohamadnia Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran. Biotechnology Department, advanced technologies in medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2016 09 15 2016 09 15 Introduction: Pemphigus comprises a group of diseases characterized by cutaneous and mucosal blistering. Pemphigus vulgaris (PV) is the most common and important variant. Recent reports have revealed the relatively high incidence of PV in Iran. The onset or aggravation of many human diseases is attributed to oxidative stress and it has been suggested as one of the several factors responsible for etiopathogenesis of pemphigus. The present study sought to assess the association of salivary level of antioxidant enzyme glutathione peroxidase (GPx) and total antioxidant level with pemphigus vulgaris. Materials and Methods: This case-control study evaluated patients referred to Razi Dermatology Hospital in Tehran , Iran from January to November 2011. Thirty patients with early diagnosed PV, who had never been on treatment were enrolled in this study. The diagnosis of PV was confirmed by histopathology and direct immunofluorescence. The control group consisted of 30 age- and sex-matched healthy non-smoker volunteers. Saliva was collected from subjects for evaluation of glutathione peroxidase and total antioxidant levels and comparison with controls. GPX and TAS activity was analyzed by spectrophotometry . Independent samples t-test was applied to compare the means of continuous variables. Statistical analysis was performed using SPSS software. P ≤ 0.05 was considered significant. Results: The mean salivary level of glutathione peroxidase was significantly lower in pemphigus patients. No statistically significant difference was observed between the groups in total antioxidant levels of saliva. Conclusion: Our findings indicated that lower levels of glutathione peroxidase may be associated with pemphigus vulgaris. Salivary GPx level may be used for diagnosis of PV. Key words: Antioxidant, Pemphigus vulgaris, Glutathione peroxidase. https://jcr.tums.ac.ir/index.php/jcr/article/view/71 https://jcr.tums.ac.ir/index.php/jcr/article/download/71/123