Osteogain as a new liquid formulation for emdogain: A review of the literature
AbstractObjective: The purpose of this study was to review the scientific evidence regarding the efficacyof Osteogain.Materials and Methods: A literature search for relevant english articles was performed till September, 2017 through PubMed and Google Scholar databases.Results: After screening the titles and abstracts, eight studies were found to be relevant and included in the study while considering the specific criteria. Studies consist of six In Vitro and two Animal studies. Significantly higher amount of total adsorbed amelogenin for Osteogain has beenreported by enzyme-linked immunosorbent assay (ELISA). Histologic evaluation showed higher amounts of connective tissue attachment and bone formation for Osteogain. Micro-CT analysis demonstrated that Osteogain induced significant new bone formation. Real-time polymerase chain reaction (PCR) revealed that Osteogain significantly upregulated the expression of the genes involved in osteogenesis and increased alizarin red staining.Conclusions: Most studies indicated promising results about the use of Osteogain in periodontal regeneration. Further investigations are needed to discover the characteristics of this novel liquid enamel matrix derivative (EMD) formulation (Osteogain).Key words: Enamel matrix derivative (EMD), Emdogain, Osteogain.
Miron RJ, Dard M and Weinreb M. Enamel matrix derivative, inflammation and soft tissue wound healing. J Periodontal Res. 2014; 50(5):555-69.
Hammarstrom L. Enamel matrix, cementum development and regeneration. J Clin Periodontol.1997; 24(9):658–68.
Polimeni G, Koo K.T, Qahash M, Xiropaidis A.V,Albandar J.M, Wikesjo U.M Prognostic factors for alveolar regeneration: effect of a space-providing biomaterial on guided tissue regeneration. J Clin Periodontol. 2004; 31(9): 725–29.
Siciliano V.I, Andreuccetti G, Siciliano A.I, Blasi A, Sculean A, Salvi G.E. Clinical outcomes after treatment of non-contained intrabony defects with enamel matrix derivative or guided tissue regeneration: a 12-month randomized controlled clinical trial. J Periodontol. 2011; 82(1): 62–71.
Aspriello SD, Ferrante L, Rubini C, Piemontese M. Comparative study of DFDBA in combination with enamel matrix derivative versus DFDBA alone for treatment of periodontal intrabony defects at 12 months post-surgery. Clin Oral Investig.2011; 15(2):225-32.
Yilmaz S, Cakar G, Yildirim B, Sculean A. Healing of two and three wall intrabony periodontal defects following treatment with an enamel matrix derivative combined with autogenous bone. J Clin Periodontol. 2010; 37(6):544-50.
Miron RJ, Wei L, Bosshardt DD, Buser D, Sculean A, Zhang Y. Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects. Clin Oral Investig. 2014; 18(2):471-78.
Hoidal MJ, Grimard BA, Mills MP, Schoolfield JD, Mellonig JT, Mealey BL. Clinical evaluation of demineralized freeze-dried bone allograft with and without enamel matrix derivative for the treatment of periodontal osseous defects in humans. JPeriodontol. 2008; 79(12):2273-80.
Guida L, Annunziata M, Belardo S, Farina R, Scabbia A, Trombelli L. Effect of autogenous cortical bone particulate in conjunction with enamel matrix derivative in the treatment of periodontal ntraosseous defects. J Periodontol. 2007;78(2):231- 38.
Miron RJ, Bosshardt D, Laugische O, Dard M, Gemperli AC, Buser D, et al. In Vitro Evaluation of Demineralized Freeze-Dried Bone Allograft in Combination With Enamel Matrix Derivative. J Periodontol. 2013; 84(11):1646-54.
Miron RJ, Bosshardt DD, Hedbom E, Zhang Y, Haenni B,Buser D, et al. Adsorption of enamel matrix proteins to a bovine-derived bone graftingmaterial and its regulation of cell adhesion, proliferation,and differentiation. J Periodontol. 2012; 83(7):936-47.
Protivinsky J, Appleford M, Strnad J, Helebrant A, Ong JL. Effect of chemically modified titanium surfaces on protein adsorption and osteoblast precursor cell behavior. International Journal of Oral and Maxillofacial Implants. 2007; 22(4):542.
Wittmer CR, Phelps JA, Saltzman WM, Van Tassel PR. Fibronectin terminated multilayer films: protein adsorption and cell attachment studies. Biomaterials.2007; 28(5):851-60.
Shirakata Y, Miron RJ, Nakamura T, Sena K, Shinohara Y, Horai N, et al. Effects of EMD-liquid (Osteogain) on periodontal healing in class III furcation defects in monkeys. J Clin Periodontol.2017 Mar; 44(3):298-307.
Zhang Y, Jing D, Buser D, Sculean A, Chandad F, Miron RJ. Bone grafting material in combination with Osteogain for bone repair: A rat histomorphometric study. Clin Oral Investig. 2016;20(3):589–95.
Miron RJ, Fujioka-Kobayashi M, Zhang Y, Caballé-Serrano J, Shirakata Y, Bosshardt DD, et al. Osteogain improves osteoblast adhesion, proliferation and differentiation on a bovine-derived natural bone mineral. Clin Oral Implants Res. 2017 Mar; 28(3):327-33.
Miron RJ, Fujioka-Kobayashi M, Zhang Y, Sculean A, Pippenger B, Shirakata Y, et al. Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro. Clin Oral Investig. 2016 Dec 1; 21(7):2265-72.
Miron RJ, Fujioka-Kobayashi M, Buser D, Zhang Y, Bosshardt DD, Sculean A. Combination of Collagen Barrier Membrane with Enamel MatrixDerivative-Liquid Improves Osteoblast Adhesion and Differentiation. Int J Oral Maxillofac Implants. 2017 Jan/Feb; 32(1):196-203.
Miron RJ, Chandad F, Buser D, Sculean A, CochranDL, Zhang Y. Effect of Enamel Matrix Derivative Liquid on Osteoblast and Periodontal Ligament Cell Proliferation and Differentiation. J Periodontol. 2016 Jan; 87(1):91-9.
Miron RJ, Bosshardt DD, Buser D, Zhang Y, Tugulu S, Gemperli A, et al. Comparison of the of enamel matrix derivative gel and enamelmatrix derivative in liq- uid formulation to adsorb to bone grafting materials. J Periodontol 2015; 86(4):578–87.
Miron RJ, Shuang Y, Sculean A, Buser D, Chandad F, Zhang Y. Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material. Clin Oral Investig. 2016 Nov; 20(8):2037-43.
Miron RJ, Caluseru OM, Guillemette V, Zhang Y, Gemperli AC, Chandad F, et al. Influence of enamel matrix derivative on cells at different maturation stages of differentiation. PLoS One. 2013;8(8): e71008.
Miron RJ, Saulacic N, Buser D, Iizuka, T, Sculean A.Osteoblast proliferation and differentiation on a barrier membrane in combination with bmp2 and tgfbeta1. Clin Oral Investig. 2013; 17(3): 981–88.
Murray G, Holden R, Roschlau W. Experimental and clinical study of new growth of bone in a cavity.Am J Surg. 1957; 93(3):385-87.
Araujo MG, Lindhe J. Ridge preservation with theuse of Bio-Oss collagen: A 6-month study in the dog. Clin Oral Implants Res 2009;20(5):433-40.
A signature of author below certifies compliance with the following statements:
Copyright transfer; in signing this Agreement:
1. I hereby warrant that this article is an original work, has not been published before and is not being considered for publication elsewhere in its final form either in printed or electronic form;
2. I hereby warrant that you have obtained permission from the copyright holder to reproduce in the Article (in all media including print and electronic form) material not owned by you, and that you have acknowledged the source;
3. I hereby warrant that this article contains no violation of any existing copyright, moral rights or other third party right or any material of an obscene, indecent, defamatory or otherwise unlawful nature and that to the best of your knowledge this Article does not infringe the rights of others;
4. I hereby warrant that in the case of a multi-authored Article you have obtained, in writing, authorization to enter into this Agreement on their behalf and that all co-authors have read and agreed the terms of this agreement;
5. I warrant that any formula or dosage given is accurate and will not if properly followed injure any person;
6. I have made a significant scientific contribution to the study and I have read the complete manuscript and take responsibility for the content and completeness of the final surmised manuscript;
7. I will indemnify and keep indemnified the Editors, the Journal's Editor against all claims and expenses (including legal costs and expenses) arising from any breach of this warranty and the other warranties on your behalf in this Agreement.
Conflict of interest disclosure
All institutional or corporeal affixations of mine and all funding sources for the study are acknowledged. I certify that I have no commercial association that might represent a conflict of interest in connection with the submitted manuscript.