Evaluation Of The Effect Of COMT Gene Polymorphism In Rs4680 On The Occurrence Of Concomitant Head, Neck And Back Pain In Patients With Temporomandibular Joint Disorders
Abstract
Background: Temporomandibular disorders (TMD) include a number of clinical conditions involving the masticatory muscles, temporomandibular joint (TMJ), and adjacent structures. The main feature of TMD is pain. According to past studies, TMD can have genetic causes. One of the genes that seems to be important in this regard is COMT. This study aims to compare the effect of COMT gene polymorphism rs4680 on the occurrence of simultaneous head, neck and back pain in patients with TMD.Methods: This study was conducted as a case-control study on patients aged between 18 and 65 years who had TMD disorder. After meeting the conditions for entering the study, a written informed consent was obtained from the people participating in this project. Then a blood sample of 5cc was prepared from each person and poured into tubes containing EDTA anticoagulant and used for DNA extraction and identification. The COMT gene was sent to the genetics laboratory and was compared in terms of the polymorphism of the COMT gene using the PCRARMS technique.Results: The sample size was 100 people aged 18 to 65 years. Variables with normal distribution were analyzed with Chi-Square test and variables with non-normal distribution with Mann-Whitney test. After genetic analysis in terms of rs4680 gene polymorphism, 18 people (18%) were identified with AA, 80 people (80%) with GA and 2 people (2%) with GG. "temporal headache in the last 30 days", "temporal headache change with chewing hard foods", "temporal headache change with opening the mouth or moving the jaw forward or to the sides", " Temporal headache changes with jaw habits such as teeth touching, pressing or grinding teeth together, chewing gum ", " Temporal headache changes with jaw activities such as talking, kissing or yawning ", " having Back pain during the last 4 weeks", having pain in the arm, leg, joints (neck) during the last 4 weeks", “headache during 4 last weeks” and "time of onset of headache in the temporal region" did not have significant relationships with COMT gene polymorphism rs4680 in patients with TMD.(repectively, p value=0.873، p value=0.658، p value=0.518 p value=0.685، p value=0.884،p value=0.654، p value=0.723، p value=0.831، p value=0.692 ).Conclusion: According to previous studies, there is a significant relationship between rs4680 polymorphism in the COMT gene and the occurrence of temporomandibular disorders (TMD), although in our study, no significant relationship was found between headache and neck and back pain with this polymorphism.
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2. 55. Michelotti, A., et al., Catechol-O-methyltransferase (COMT) gene polymorphisms as risk factor in temporomandibular disorders patients from Southern Italy. The Clinical journal of pain, 2014. 30(2): p. 129-133.
3. MacGregor AJ, Andrew T, Sambrook PN, Spector TD. Structural, psychological, and genetic influences on low back and neck pain: a study of adult female twins. Arthritis Rheum. 2004;51(2):160-7.
4. Tammimäki A, Männistö PT. Catechol-O-methyltransferase gene polymorphism and chronic human pain: a systematic review and meta-analysis. Pharmacogenet Genomics. 2012;22(9):673-91.
5. Lotta T, Vidgren J, Tilgmann C, Ulmanen I, Melén K, Julkunen I, et al. Kinetics of human soluble and membrane-bound catechol O-methyltransferase: a revised mechanism and description of the thermolabile variant of the enzyme. Biochemistry. 1995;34(13):4202-10.
6. Mladenovic I, Supic G, Kozomara R, Dodic S, Ivkovic N, Milicevic B, et al. Genetic Polymorphisms of Catechol-O-Methyltransferase: Association with Temporomandibular Disorders and Postoperative Pain. J Oral Facial Pain Headache. 2016;30(4):302-10.
7. Marbach, J.J. and M. Levitt, Erythrocyte catechol-O-methyltransferase activity in facial pain patients. Journal of dental research, 1976. 55(4): p. 711-711.
8. Antonini, A., et al., COMT inhibition with tolcapone in the treatment algorithm of patients with Parkinson’s disease (PD): relevance for motor and non-motor features. Neuropsychiatric disease and treatment, 2008. 4(1): p. 1.
9. Männistö PT, Kaakkola S. Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors. Pharmacol Rev. 1999;51(4):593-628.
10. Mladenovic, I., et al., Genetic Polymorphisms of Catechol-O-Methyltransferase: Association with Temporomandibular Disorders and Postoperative Pain. Journal of Oral & Facial Pain & Headache, 2016. 30(4).
11. Hagen K, Pettersen E, Stovner LJ, Skorpen F, Zwart JA. The association between headache and Val158Met polymorphism in the catechol-O-methyltransferase gene: the HUNT Study. J Headache Pain. 2006;7(2):70-4.
12. Park, J.W., et al., Genetic contribution of catechol-O-methyltransferase polymorphism in patients with migraine without aura. Journal of Clinical Neurology, 2007. 3(1): p. 24-30.
13. Omair A, Lie BA, Reikeras O, Holden M, Brox JI. Genetic contribution of catechol-O-methyltransferase variants in treatment outcome of low back pain: a prospective genetic association study. BMC Musculoskeletal Disorders. 2012;13(1):76.
14. Zubieta JK, Heitzeg MM, Smith YR, Bueller JA, Xu K, Xu Y, et al. COMT val158met genotype affects mu-opioid neurotransmitter responses to a pain stressor. Science. 2003;299(5610):1240-3.
15. Erdal ME, Herken H, Mutlu MN, Bayazit YA. Significance of catechol-O-methyltransferase gene polymorphism in myofacial pain syndrome. The Pain Clinic. 2003;15(3):309-13.
16. Najafi S, Gholizadeh N, Roudgari H, Sheykhbahaei N, Oloumi AM. Temporomandibular disorders is associated with genetic factors: A review. Journal of Craniomaxillofacial Research. 2022 Nov 19:69-80.
17. Marbach JJ, Levitt M. Erythrocyte catechol-O-methyltransferase activity in facial pain patients. J Dent Res. 1976;55(4):711.
2. 55. Michelotti, A., et al., Catechol-O-methyltransferase (COMT) gene polymorphisms as risk factor in temporomandibular disorders patients from Southern Italy. The Clinical journal of pain, 2014. 30(2): p. 129-133.
3. MacGregor AJ, Andrew T, Sambrook PN, Spector TD. Structural, psychological, and genetic influences on low back and neck pain: a study of adult female twins. Arthritis Rheum. 2004;51(2):160-7.
4. Tammimäki A, Männistö PT. Catechol-O-methyltransferase gene polymorphism and chronic human pain: a systematic review and meta-analysis. Pharmacogenet Genomics. 2012;22(9):673-91.
5. Lotta T, Vidgren J, Tilgmann C, Ulmanen I, Melén K, Julkunen I, et al. Kinetics of human soluble and membrane-bound catechol O-methyltransferase: a revised mechanism and description of the thermolabile variant of the enzyme. Biochemistry. 1995;34(13):4202-10.
6. Mladenovic I, Supic G, Kozomara R, Dodic S, Ivkovic N, Milicevic B, et al. Genetic Polymorphisms of Catechol-O-Methyltransferase: Association with Temporomandibular Disorders and Postoperative Pain. J Oral Facial Pain Headache. 2016;30(4):302-10.
7. Marbach, J.J. and M. Levitt, Erythrocyte catechol-O-methyltransferase activity in facial pain patients. Journal of dental research, 1976. 55(4): p. 711-711.
8. Antonini, A., et al., COMT inhibition with tolcapone in the treatment algorithm of patients with Parkinson’s disease (PD): relevance for motor and non-motor features. Neuropsychiatric disease and treatment, 2008. 4(1): p. 1.
9. Männistö PT, Kaakkola S. Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors. Pharmacol Rev. 1999;51(4):593-628.
10. Mladenovic, I., et al., Genetic Polymorphisms of Catechol-O-Methyltransferase: Association with Temporomandibular Disorders and Postoperative Pain. Journal of Oral & Facial Pain & Headache, 2016. 30(4).
11. Hagen K, Pettersen E, Stovner LJ, Skorpen F, Zwart JA. The association between headache and Val158Met polymorphism in the catechol-O-methyltransferase gene: the HUNT Study. J Headache Pain. 2006;7(2):70-4.
12. Park, J.W., et al., Genetic contribution of catechol-O-methyltransferase polymorphism in patients with migraine without aura. Journal of Clinical Neurology, 2007. 3(1): p. 24-30.
13. Omair A, Lie BA, Reikeras O, Holden M, Brox JI. Genetic contribution of catechol-O-methyltransferase variants in treatment outcome of low back pain: a prospective genetic association study. BMC Musculoskeletal Disorders. 2012;13(1):76.
14. Zubieta JK, Heitzeg MM, Smith YR, Bueller JA, Xu K, Xu Y, et al. COMT val158met genotype affects mu-opioid neurotransmitter responses to a pain stressor. Science. 2003;299(5610):1240-3.
15. Erdal ME, Herken H, Mutlu MN, Bayazit YA. Significance of catechol-O-methyltransferase gene polymorphism in myofacial pain syndrome. The Pain Clinic. 2003;15(3):309-13.
16. Najafi S, Gholizadeh N, Roudgari H, Sheykhbahaei N, Oloumi AM. Temporomandibular disorders is associated with genetic factors: A review. Journal of Craniomaxillofacial Research. 2022 Nov 19:69-80.
17. Marbach JJ, Levitt M. Erythrocyte catechol-O-methyltransferase activity in facial pain patients. J Dent Res. 1976;55(4):711.
| Files | ||
| Issue | Vol 10, No 4 (Autumn 2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/jcr.v10i4.15305 | |
| Keywords | ||
| Keywords: Temporomandibular joint disorder headache COMT gene rs4680 | ||
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How to Cite
1.
Najafi S, Roudgari H, Sheykhbahaei N, Oloumi AM. Evaluation Of The Effect Of COMT Gene Polymorphism In Rs4680 On The Occurrence Of Concomitant Head, Neck And Back Pain In Patients With Temporomandibular Joint Disorders. J Craniomaxillofac Res. 2024;10(4):146-152.
