Invasive Mucormycosis and the Importance of Molecular Diagnostics
-
Abdolreza Mohamadnia
,
Amir Jalal Abassi
,
Farnoush Mohammadi
,
Seyedeh Zahra Fotook Kiaei
,
Mona Mohajeri Tehrani
,
Somaieh Lookzadeh
,
Fatemeh Yassari
,
Shadi Shafaghi
,
Farnaz Ahmadi
,
Naghmeh Bahrami
Abstract
Introduction: Mucormycosis, an infection, with a high death rate, requires understanding its symptoms, diagnosis methods and treatment options due, to its growing occurrence. The research employs a molecular method to evaluate the diagnosis of the mucormycosis. Materials and Methods: In this study, we obtained 30 samples from patients undergoing diagnosis and conducted DNA extraction. Additionally, DNA extraction was carried out on 30 tissue samples suspected of infection in paraffin blocks. Subsequently, PCR and Real-time PCR were performed using targeted primers, for mucormycosis followed by analysis of the results. Results: In the research findings among 30 liquid samples 8 tested positive, for mucor using the PCR method. 10 using the Real-time PCR method. Similarly, out of 30 tissue samples, 9 cases showed mucor presence with the PCR method and 11 cases, with the Realtime PCR method. Conclusion: In this study, real-time PCR and PCR techniques showed promising and faster results in detecting Mucor than the culture approach. The molecular methods provided results that could be of great use for scenarios. Keywords: Mucormycosis; Diagnosis; Real-time PCR.
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10. Bahrami N, Poorahmad M, Hosseini F, Mohammadi F, Rasouli A, Tehrani MM, et al. Comparing Salivary Mir-125 And Mir-30 Expression In Oral Squamous Cell Carcinoma To Healthy Individuals. Journal of Craniomaxillofacial Research. 2023:162-6.
11. Karimi R, Mohamadnia A, Hosseini F, Bahrami N, Jamaati H. Evaluation of Chemokines and Cytokines as Biomarkers for Disease Severity in COVID-19-Infected Patients. Jundishapur Journal of Microbiology. 2024;17(1).
12. Emami N, Bahrami N, Mirzaei M, Mohamadnia A. Gene expression of one important microRNA in blood and tissue samples of oral squamous cell carcinoma. Journal of Craniomaxillofacial Research. 2020:132-7.
13. Rouzbahani H, Badiee P, Barkhordar M, Badiee A, Faramarzi A, Moghadami M, et al. Etiologic Causes of Invasive Fungal Sinusitis among Patients of, University Hospitals in Shiraz, Iran. Journal of Isfahan Medical School. 2011;29(153):1286-96.
14. Stemler J, Többen C, Lass-Flörl C, Steinmann J, Ackermann K, Rath P-M, et al. Diagnosis and Treatment of Invasive Aspergillosis Caused by Non-fumigatus Aspergillus spp. Journal of Fungi. 2023;9(4):500.
15. Rasouli A, Arzi L, Khademi S, Mohamadnia A. Assessment of miR-182, miR-221 and CEA Expression in the Peripheral Blood of Oral Squamous Cell Carcinoma (OSCC) Patients. Journal of Craniomaxillofacial Research. 2023.
16. Kazempour Dizaji M, Farzanegan B, Bahrami N, Khoshnam Z, Fathi M, Dargahi H, et al. Expression of miRNA1, miRNA133, miRNA191, and miRNA24, as good biomarkers, in non-small cell lung cancer using real-time PCR method. Asian Pacific Journal of Cancer Prevention. 2022;23(5):1565-70.
17. Emami N, Mohamadnia A, Mirzaei M, Bayat M, Mohammadi F, Bahrami N. miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers. Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2020;46(5):341-7.
18. Shadrivova OV, Burygina EV, Klimko NN. Molecular diagnostics of mucormycosis in hematological patients: a literature review. Journal of Fungi. 2019;5(4):112.
19. Skiada A, Pagano L, Groll A, Zimmerli S, Dupont B, Lagrou K, et al. Zygomycosis in Europe: analysis of 230 cases accrued by the registry of the European Confederation of Medical Mycology (ECMM) Working Group on Zygomycosis between 2005 and 2007. Clinical Microbiology and Infection. 2011;17(12):1859-67.
20. Millon L, Herbrecht R, Grenouillet F, Morio F, Alanio A, Letscher-Bru V, et al. Early diagnosis and monitoring of mucormycosis by detection of circulating DNA in serum: retrospective analysis of 44 cases collected through the French Surveillance Network of Invasive Fungal Infections (RESSIF). Clinical Microbiology and Infection. 2016;22(9):810. e1-. e8.
21. Scherer E, Iriart X, Bellanger AP, Dupont D, Guitard J, Gabriel F, et al. Quantitative PCR (qPCR) detection of Mucorales DNA in bronchoalveolar lavage fluid to diagnose pulmonary mucormycosis. Journal of Clinical Microbiology. 2018;56(8):10.1128/jcm. 00289-18.
22. Hammond SP, Bialek R, Milner DA, Petschnigg EM, Baden LR, Marty FM. Molecular methods to improve diagnosis and identification of mucormycosis. Journal of Clinical Microbiology. 2011;49(6):2151-3.
2. Lanternier F, Dannaoui E, Morizot G, Elie C, Garcia-Hermoso D, Huerre M, et al. A global analysis of mucormycosis in France: the RetroZygo Study (2005–2007). Clinical Infectious Diseases. 2012;54(suppl_1):S35-S43.
3. Kontoyiannis DP, Marr KA, Park BJ, Alexander BD, Anaissie EJ, Walsh TJ, et al. Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients, 2001–2006: overview of the Transplant-Associated Infection Surveillance Network (TRANSNET) Database. Clinical Infectious Diseases. 2010;50(8):1091-100.
4. Slavin M, Van Hal S, Sorrell T, Lee A, Marriott D, Daveson K, et al. Invasive infections due to filamentous fungi other than Aspergillus: epidemiology and determinants of mortality. Clinical Microbiology and Infection. 2015;21(5):490. e1-. e10.
5. Bahrami N, Jamaati H, Farzanegan B, Nejad RA, Mohammadi F, Naghdi M, et al. Special Real-Time PCR and Conventional PCR for Detection of Rhino-Orbital-Cerebral Mucormycosis. Journal of Cellular & Molecular Anesthesia. 2024;9(1).
6. Patterson TF, Donnelly JP. New concepts in diagnostics for invasive mycoses: non-culture-based methodologies. Journal of Fungi. 2019;5(1):9.
7. von Hertwig AM, Sant'Ana AS, Sartori D, da Silva JJ, Nascimento MS, Iamanaka BT, et al. Real-time PCR-based method for rapid detection of Aspergillus niger and Aspergillus welwitschiae isolated from coffee. Journal of microbiological methods. 2018;148:87-92.
8. Kontoyiannis DP, Lewis RE. Agents of mucormycosis and entomophthoramycosis. Mandell, Douglas, and Bennett's Principles and practice of infectious diseases. 2010:3257-69.
9. Barnes RA, White PL, Morton CO, Rogers TR, Cruciani M, Loeffler J, et al. Diagnosis of aspergillosis by PCR: clinical considerations and technical tips. Medical mycology. 2018;56(suppl_1):S60-S72.
10. Bahrami N, Poorahmad M, Hosseini F, Mohammadi F, Rasouli A, Tehrani MM, et al. Comparing Salivary Mir-125 And Mir-30 Expression In Oral Squamous Cell Carcinoma To Healthy Individuals. Journal of Craniomaxillofacial Research. 2023:162-6.
11. Karimi R, Mohamadnia A, Hosseini F, Bahrami N, Jamaati H. Evaluation of Chemokines and Cytokines as Biomarkers for Disease Severity in COVID-19-Infected Patients. Jundishapur Journal of Microbiology. 2024;17(1).
12. Emami N, Bahrami N, Mirzaei M, Mohamadnia A. Gene expression of one important microRNA in blood and tissue samples of oral squamous cell carcinoma. Journal of Craniomaxillofacial Research. 2020:132-7.
13. Rouzbahani H, Badiee P, Barkhordar M, Badiee A, Faramarzi A, Moghadami M, et al. Etiologic Causes of Invasive Fungal Sinusitis among Patients of, University Hospitals in Shiraz, Iran. Journal of Isfahan Medical School. 2011;29(153):1286-96.
14. Stemler J, Többen C, Lass-Flörl C, Steinmann J, Ackermann K, Rath P-M, et al. Diagnosis and Treatment of Invasive Aspergillosis Caused by Non-fumigatus Aspergillus spp. Journal of Fungi. 2023;9(4):500.
15. Rasouli A, Arzi L, Khademi S, Mohamadnia A. Assessment of miR-182, miR-221 and CEA Expression in the Peripheral Blood of Oral Squamous Cell Carcinoma (OSCC) Patients. Journal of Craniomaxillofacial Research. 2023.
16. Kazempour Dizaji M, Farzanegan B, Bahrami N, Khoshnam Z, Fathi M, Dargahi H, et al. Expression of miRNA1, miRNA133, miRNA191, and miRNA24, as good biomarkers, in non-small cell lung cancer using real-time PCR method. Asian Pacific Journal of Cancer Prevention. 2022;23(5):1565-70.
17. Emami N, Mohamadnia A, Mirzaei M, Bayat M, Mohammadi F, Bahrami N. miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers. Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2020;46(5):341-7.
18. Shadrivova OV, Burygina EV, Klimko NN. Molecular diagnostics of mucormycosis in hematological patients: a literature review. Journal of Fungi. 2019;5(4):112.
19. Skiada A, Pagano L, Groll A, Zimmerli S, Dupont B, Lagrou K, et al. Zygomycosis in Europe: analysis of 230 cases accrued by the registry of the European Confederation of Medical Mycology (ECMM) Working Group on Zygomycosis between 2005 and 2007. Clinical Microbiology and Infection. 2011;17(12):1859-67.
20. Millon L, Herbrecht R, Grenouillet F, Morio F, Alanio A, Letscher-Bru V, et al. Early diagnosis and monitoring of mucormycosis by detection of circulating DNA in serum: retrospective analysis of 44 cases collected through the French Surveillance Network of Invasive Fungal Infections (RESSIF). Clinical Microbiology and Infection. 2016;22(9):810. e1-. e8.
21. Scherer E, Iriart X, Bellanger AP, Dupont D, Guitard J, Gabriel F, et al. Quantitative PCR (qPCR) detection of Mucorales DNA in bronchoalveolar lavage fluid to diagnose pulmonary mucormycosis. Journal of Clinical Microbiology. 2018;56(8):10.1128/jcm. 00289-18.
22. Hammond SP, Bialek R, Milner DA, Petschnigg EM, Baden LR, Marty FM. Molecular methods to improve diagnosis and identification of mucormycosis. Journal of Clinical Microbiology. 2011;49(6):2151-3.
| Files | ||
| Issue | Vol 11, No 2 (Spring 2024) | |
| Section | Original Article(s) | |
| Keywords | ||
| Mucormycosis; Diagnosis; Real-time PCR. | ||
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How to Cite
1.
Mohamadnia A, Abassi AJ, Mohammadi F, Fotook Kiaei SZ, Mohajeri Tehrani M, Lookzadeh S, Yassari F, Shafaghi S, Ahmadi F, Bahrami N. Invasive Mucormycosis and the Importance of Molecular Diagnostics. J Craniomaxillofac Res. 2024;11(2):87-92.
