In Vitro Comparative Analysis of Antioxidant Activities in Ethanolic Extracts of Centella Asiatica Linn., Ocimum Basilicum, Ocimum Sanctum, and Camellia Sinensis
-
Nazifa Javaid
,
Meghanand T Nayak
,
Anjali Nayak
,
Rezhat Abass
,
Mohd. Zanul Abedeen
,
Milind Sharad Pande
Abstract
Introduction: To comparatively evaluate the antioxidant potential of ethanolic extracts of Centella asiatica Linn. (CA), Ocimum basilicum Linn. (OB), Ocimum sanctum (OS), and Camellia sinensis (CS) using multiple in vitro assays. Materials and Methods: Ethanolic extracts of CA, OB, OS, and CS were prepared and subjected to in vitro antioxidant analysis, including total antioxidant capacity, DPPH radical scavenging, nitric oxide radical scavenging, reducing power, and superoxide anion radical scavenging assays using UV spectrophotometry. Antioxidant activities were recorded at concentrations up to 1000 µg/ml. All assays were performed across a concentration range of 10–1000 µg/ml using serial dilutions. Results: Total antioxidant activity (109.40 ± 0.15 % inhibition), DPPH scavenging (107.88 ± 9.32 % inhibition), nitric oxide scavenging (98.27 ± 9.48 % inhibition), reducing power (absorbance: 93.12 ± 9.12 at 700 nm), and superoxide scavenging (88.93 ± 8.14 % inhibition). The differences between groups were statistically significant (p < 0.001). Conclusion: CA demonstrated superior antioxidant activity compared to OB, OS, and CS. Its high antioxidant potential suggests its promise as a natural therapeutic agent for preventing oxidative stress–related diseases.Keywords: Antioxidants; Centella asiatica; Camellia sinensis; Ocimum basilicum; Ocimum sanctum; Oxidative stress.
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7. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26.
8. Padmiswari AIM, Wulansari NT, Indrayoni P. Antioxidant activity test of combination of Centella asiatica leaf extract and mint leaf extract as an alternative herbal drink. Jurnal Pijar Mipa. 2023;18(1):126–9.
9. Roy Z, Bansal R, Siddiqui L, Chaudhary N. Understanding the Role of Free Radicals and Antioxidant Enzymes in Human Diseases. Current Pharmaceutical Biotechnology. 2023;24(10):1265-1276.
10. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763.
11. Nimse S, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015;5:27986–8006.
12. Forman HJ, Zhang H. Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nat Rev Drug Discov. 2021;20(9):689-709.
13. Zafar F, Asif HM, Shaheen G, Ghauri AO, Rajpoot SR, Tasleem MW, et al. A comprehensive review on medicinal plants possessing antioxidant potential. Clin Exp Pharmacol Physiol. 2023;50(3):205-217.
14. Pittella F, Dutra RC, Junior DD, Lopes MTP, Barbosa NR. Antioxidant and cytotoxic activities of Centella asiatica (L) Urb. Int J Mol Sci. 2009;10(9):3713-3721.
15. Brinkhaus B, Lindner M, Schuppan D, Hahn EG. Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine. 2000;7(5):427-48.
16. Ullah MO, Sultana S, Haque A, Tasmin S, Antimicrobial, Cytotoxic and Antioxidant Activity of Centella asiatica. Eur J Sci Res. 2009;30:260-4.
17. Tariq AL, Reyaz AL. Antioxidant activity of Camellia sinensis leaves. Int J Curr Microbiol App Sci 2013;2:40-6.
18. Eid AM, Jaradat N, Shraim N, et al. Assessment of anticancer, antimicrobial, antidiabetic, anti-obesity and antioxidant activity of Ocimum Basilicum seeds essential oil from Palestine. BMC Complement Med Ther. 2023;23, 221
2. Polash SA, et.al. Phytochemical contents, antioxidant and antibacterial activity of ethanolic extracts of Centella asiatica (L.) Urb. leaf and stem. Jahangirnagar University J. Biol. Sci. 2017;6:51-7.
3. Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014;94(2):329-54.
4. Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, et al. Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Front Physiol. 2020;11:694.
5. Adtani P, et.al. In-vitro antioxidant activity of ethanolic extracts of Centella asiatica L., Oregano vulgare sub. sp hirtum and Ocimum basilicum L. via five model systems. Ind J Res Pharmacy Biotechnol 2014;2:1230-6.
6. Gupta N, Verma K, Nalla S, Kulshreshtha A, Lall R, Prasad S. Free Radicals as a Double-Edged Sword: The Cancer Preventive and Therapeutic Roles of Curcumin. Molecules. 2020;25(22):5390.
7. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26.
8. Padmiswari AIM, Wulansari NT, Indrayoni P. Antioxidant activity test of combination of Centella asiatica leaf extract and mint leaf extract as an alternative herbal drink. Jurnal Pijar Mipa. 2023;18(1):126–9.
9. Roy Z, Bansal R, Siddiqui L, Chaudhary N. Understanding the Role of Free Radicals and Antioxidant Enzymes in Human Diseases. Current Pharmaceutical Biotechnology. 2023;24(10):1265-1276.
10. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763.
11. Nimse S, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015;5:27986–8006.
12. Forman HJ, Zhang H. Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nat Rev Drug Discov. 2021;20(9):689-709.
13. Zafar F, Asif HM, Shaheen G, Ghauri AO, Rajpoot SR, Tasleem MW, et al. A comprehensive review on medicinal plants possessing antioxidant potential. Clin Exp Pharmacol Physiol. 2023;50(3):205-217.
14. Pittella F, Dutra RC, Junior DD, Lopes MTP, Barbosa NR. Antioxidant and cytotoxic activities of Centella asiatica (L) Urb. Int J Mol Sci. 2009;10(9):3713-3721.
15. Brinkhaus B, Lindner M, Schuppan D, Hahn EG. Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine. 2000;7(5):427-48.
16. Ullah MO, Sultana S, Haque A, Tasmin S, Antimicrobial, Cytotoxic and Antioxidant Activity of Centella asiatica. Eur J Sci Res. 2009;30:260-4.
17. Tariq AL, Reyaz AL. Antioxidant activity of Camellia sinensis leaves. Int J Curr Microbiol App Sci 2013;2:40-6.
18. Eid AM, Jaradat N, Shraim N, et al. Assessment of anticancer, antimicrobial, antidiabetic, anti-obesity and antioxidant activity of Ocimum Basilicum seeds essential oil from Palestine. BMC Complement Med Ther. 2023;23, 221
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| Issue | Vol 13, No 1 (Winter 2026) | |
| Section | Original Article(s) | |
| Keywords | ||
| Antioxidants; Centella asiatica; Camellia sinensis; Ocimum basilicum; Ocimum sanctum; Oxidative stress. | ||
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How to Cite
1.
Javaid N, T Nayak M, Nayak A, Abass R, Abedeen MZ, Pande MS. In Vitro Comparative Analysis of Antioxidant Activities in Ethanolic Extracts of Centella Asiatica Linn., Ocimum Basilicum, Ocimum Sanctum, and Camellia Sinensis. J Craniomaxillofac Res. 2026;13(1):62-69.
