Effects of FGF on tooth movement and root resorption
Abstract
Introduction: Basic fibroblast growth factor (bFGF) is an effective cytokine in angiogenesis and bone remodeling. The aim of this study was to determine the effect of locally injected bFGF on movement rate and root resorption during orthodontic force application in dogs. Materials and Methods: In this experimental animal study, four 10-12 months male dogs were selected and were randomly allocated into two groups. A week following insertion of closing coil spring between the canines and second premolars, 50μg of bFGF was injected to the case group and phosphate saline to the control group once per month. One month after second injection, the distance between the mentioned teeth were measured. Also, root resorption percentage were analyzed on the second premolars. Results: Average maxillary tooth movement rate in case and control groups were found 2.53 and 1.35mm/month respectively. The correspondent mandibular measures were 2.23mm and 1.15mm. These differences were statistically significant (P<0.0001). In respect to root resorption, the differences were found statistically significant (P<0.01) for coronal region in maxilla, which was higher in control group. Regarding the apical and middle regions of both jaws as well as coronal region of mandible the differences were insignificant. Conclusion: The results of the study showed that the local injection of bFGF can increase tooth movement rate and has the potential to decrease root resorption during orthodontic tooth movement in dogs. Keywords: Tooth movement; Root resorption; Fibroblast growth factor.
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11. Ripamanti U, Reddi H. Tissue engineering, morphogenesis, and regeneration of the periodontal tissues by bone morphogenic proteins. Crit Rev Oral Biol Med 1997;8(2):154-163.
12. Murakami S, Takayama S, Ikezawa K, et al. Regeneration of periodontal tissues by basic fibroblast growth factor. J Periodontal Res 1999;34(7):425-430.
13. King GN, Hughes FJ. Bone morphogenic protein-2 stimulates cell recruitment and cementogenesis during early wound healing. J Clin Periodontol 2001; 28(5):465-475.
14. Mellonig JT, Valderrama P, Gregory HJ, Chocrane DL. Clinical and histologic evaluation of non-surgical periodontal therapy with enamel matrix derivative: a report of four cases. J Periodontal 2009;80(9):1534-1540.
15. Qu D, Li J, Li Y, Gao Y, Zuo Y, Hsu J. Angiogenesis and osteogenesis enhanced by bFGF ex vivo gene therapy for bone tissue engineering in reconstruction of calvarial defects. J Biomed Mater Res 2011;96(3):543-551.
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17. Sako E, Hosomichi J. Alteration of bFGF expression with growth and age in ratmolar periodontal ligament. Angle Orthod 2010;80(5):904-911.
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19. Williems WF, Larsen M, Friedrich PF, Shogren KL, Bishop AT. Induction of angiogenesis and osteogenesis in surgically revascularized frozen bone allografts by sustained delivery of FGF-2 and VEGF. J Orthop Res 2012;30(10):1556-62.
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22. Seifi M,Badiei MR,Abdolazimi Z,Amdjadi P. Effects of basic fibroblast growth factor on orthodontic tooth movement in rats. Cell Journal 2013;15(3):230-237
23. Wu Lp, Liu Tt. Influence of combinative application of bFGF and IGF-1 upon periodontium remodeling in orthodontic tooth movement in rats. Heilongjiang Medicine And Pharmacy 2009; 29(32): 16-17.
24. Liao Z, Chung-Geng Z, Yin-Sheng M, Jun Z. Expressions of bFGF in rabbits’ periodontium under different orthodontic forces. Journal of Shandong University Health Sciences 2010;48(1):34-37.
25. Tang X, Liu J. Changes of basic fibroblast growth factor in periodontal tissue during orthodontic tooth movement in young rat. Journal of Jinan University of Natural Science and Medicine 2005;26(6):760-765.
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28. Brudvik P, Rygh P. Root resorption beneath the main hyaline. Eur J Orthod 1994Aug;16(4):249-263.
29. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13(6):474-484.
30. Bosshardt DD, Schroeder HE.Cementogenesis reviewed: a comparison between human premolars and rodent molars. Anat Rec 1996 June;245(2): 267-292.
31. Gotz W, Lossdorfer S, Kruger U, Braumann B, Jager A. Immunohistochemical localization of insulin-like growth factor – II and its binding protein-6 in human epithelial cells of Malassez. Eur J Oral Sci 2003 Feb;111(1):26-33.
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33. Thom SR. Oxidative stress is fundamental to hyperbaric oxygen therapy. J Appl Physiol . 2009;106(3):988-995.
34. Frantz S, Vincent KA, Feron O, Kelly RA. Innate immunity and angiogenesis. Circ Res. 2005;96(1):15-26.
35.Fozen KM, Thom SR. Hyperbaric Oxygen, Vasculogenic Stem Cells, and Wound Healing. Antioxid.Redox Signal 2014;21(11):1634–1647.
36. Giamarellos-Bourboulis EJ, Kanellakopoulou K, Pelekanou A, Tsaganos T, Kotzampassi K. Kinetics of angiopoietin-2 in serum of multi-trauma patients: correlation with patient severity. Cytokine. 2008;44(2):310-313.
37. Du W, Hu JK, Du W, Klein OD. Lineage tracing of epithelial cells in developing teeth reveals two strategies for building signaling centers. J Biol Chem. 2017;292(36):15062-15069.
38. Simon MP, Tournaire R, Pouyssegur J. The angiopoietin-2 gene of endothelial cells is up-regulated in hypoxia by a HIF binding site located in its first intron and by the central factors GATA-2 and Ets-1. J Cell Physiol. 2008;217(3):809-818.
39. Maddaluno L, Urwyler C, Werner S. Fibroblast growth factors: Key players in regeneration and tissue repair. Development 2017;144:4047-4060.
40. Kim R, Green JBA, Klein OD. From snapshots to movies: Understanding early tooth development in four dimensions. Dev Dyn. 2017;246(6):442-450.
2. Ramanathan C, Hofman Z. Root resorption in relation to orthodontic tooth movement. ActaMedica (Hradec Kralove) 2006; 49(2): 91-95.
3. Weltman B, Vig KW, Fields HW, Shanker S, Kaizar EE. Root resorption associated with orthodontic tooth movement: a systematic review. Am J Orthod Dentofacial Orthop 2010; 137(4): 462-476.
4.Kurol J, Owman-Moll P. Hyalinization and root resorption during early orthodontic tooth movement in adolescents. Angle Orthod 1998; 68(2): 161-165.
5. OwmanMoll P, Kurol J. Root resorption after orthodontic treatment in high and low-risk patients: analysis of allergy as a possible predisposing factor. Eur J Orthod 2000; 22(6): 657-663.
6. Andrade Jr I, Taddei S, Souza P. Inflammation and Tooth Movement: The Role of Cytokines, Chemokines, and Growth Factors. Seminars in Orthodontics 2012;18:257–269.
7. Krishnan V, Davidovitch Z. On a path to unfolding the biological mechanisms of orthodontic tooth movement. Journal of Dental Research 2009;88:597-608.
8. Weiland F. External root resorption and orthodontic forces: correlations and clinical consequences. Prog Orthod 2006;7(2):156-163.
9. Graber LW, Vanarsdall RL, Vig KW, Huang GJ. Orthodontics Current Principle and Techniques. 6th ed. Philadelphia: Elsevier; 2017. p. 60-64.
10. Proffit WR, Fields HW, Larson BE, Sarver DM. Contemporary Orthodontics. 6th ed. St. Louis: Elsevier; 2019. p. 269-271.
11. Ripamanti U, Reddi H. Tissue engineering, morphogenesis, and regeneration of the periodontal tissues by bone morphogenic proteins. Crit Rev Oral Biol Med 1997;8(2):154-163.
12. Murakami S, Takayama S, Ikezawa K, et al. Regeneration of periodontal tissues by basic fibroblast growth factor. J Periodontal Res 1999;34(7):425-430.
13. King GN, Hughes FJ. Bone morphogenic protein-2 stimulates cell recruitment and cementogenesis during early wound healing. J Clin Periodontol 2001; 28(5):465-475.
14. Mellonig JT, Valderrama P, Gregory HJ, Chocrane DL. Clinical and histologic evaluation of non-surgical periodontal therapy with enamel matrix derivative: a report of four cases. J Periodontal 2009;80(9):1534-1540.
15. Qu D, Li J, Li Y, Gao Y, Zuo Y, Hsu J. Angiogenesis and osteogenesis enhanced by bFGF ex vivo gene therapy for bone tissue engineering in reconstruction of calvarial defects. J Biomed Mater Res 2011;96(3):543-551.
16. Derringer KA, Linden RWA. Vascular endothelial growth factor, fibroblast growth factor 2, platelet derived growth factor and transforming growth factor beta released in human dental pulp following orthodontic forces. Archives of Oral Biology 2004;49(8):631-641.
17. Sako E, Hosomichi J. Alteration of bFGF expression with growth and age in ratmolar periodontal ligament. Angle Orthod 2010;80(5):904-911.
18. Depprich AR. Biomolecule use in tissue engineering. 1st Ed. Berlin: Springer. 2009.p.121-135.
19. Williems WF, Larsen M, Friedrich PF, Shogren KL, Bishop AT. Induction of angiogenesis and osteogenesis in surgically revascularized frozen bone allografts by sustained delivery of FGF-2 and VEGF. J Orthop Res 2012;30(10):1556-62.
20. Feito MJ, Lozano RM, Alcaide M, Ramirez-Santillan C, Arcos D, Vallet-RegiM. Immobilization and bioactivity evaluation of FGF-1 and FGF-2 on powdered silicon-doped hydroxyapatite and their scaffolds for bone tissue engineering. J Mater Sci Mater Med 2011;22(2):405-416.
21. Okada-Ban M, Thiery JP, Jouanneau J. Fibroblast growth factor-2. Int J Biochem Cell Biol 2000;32(3):263-267.
22. Seifi M,Badiei MR,Abdolazimi Z,Amdjadi P. Effects of basic fibroblast growth factor on orthodontic tooth movement in rats. Cell Journal 2013;15(3):230-237
23. Wu Lp, Liu Tt. Influence of combinative application of bFGF and IGF-1 upon periodontium remodeling in orthodontic tooth movement in rats. Heilongjiang Medicine And Pharmacy 2009; 29(32): 16-17.
24. Liao Z, Chung-Geng Z, Yin-Sheng M, Jun Z. Expressions of bFGF in rabbits’ periodontium under different orthodontic forces. Journal of Shandong University Health Sciences 2010;48(1):34-37.
25. Tang X, Liu J. Changes of basic fibroblast growth factor in periodontal tissue during orthodontic tooth movement in young rat. Journal of Jinan University of Natural Science and Medicine 2005;26(6):760-765.
26. Shiratani S, Ota M, Fujita T, Seshima F, Yamada S, Saito A. Effect of basicfibroblast growth factor on root resorption after delayed autotransplantation of tooth in dogs. Oral Surg Oral Med Oral Pathol Oral Radiol 2012 Aug;114(2):e14-21.
27. Acar A, CanyurekU. Discontinuous force application and root resorption. Angle orthod 1999; 69:159-63.
28. Brudvik P, Rygh P. Root resorption beneath the main hyaline. Eur J Orthod 1994Aug;16(4):249-263.
29. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13(6):474-484.
30. Bosshardt DD, Schroeder HE.Cementogenesis reviewed: a comparison between human premolars and rodent molars. Anat Rec 1996 June;245(2): 267-292.
31. Gotz W, Lossdorfer S, Kruger U, Braumann B, Jager A. Immunohistochemical localization of insulin-like growth factor – II and its binding protein-6 in human epithelial cells of Malassez. Eur J Oral Sci 2003 Feb;111(1):26-33.
32. Feizbakhsh M, Mortazavi MS, Razavi SM, Hajhashemi V. The effects of local injection of simvastatin on tooth movement and root resorption rates under orthodontic forces in dogs. Biosciences, Biotechnology Research Asia. 2014;11(2), 869–873.
33. Thom SR. Oxidative stress is fundamental to hyperbaric oxygen therapy. J Appl Physiol . 2009;106(3):988-995.
34. Frantz S, Vincent KA, Feron O, Kelly RA. Innate immunity and angiogenesis. Circ Res. 2005;96(1):15-26.
35.Fozen KM, Thom SR. Hyperbaric Oxygen, Vasculogenic Stem Cells, and Wound Healing. Antioxid.Redox Signal 2014;21(11):1634–1647.
36. Giamarellos-Bourboulis EJ, Kanellakopoulou K, Pelekanou A, Tsaganos T, Kotzampassi K. Kinetics of angiopoietin-2 in serum of multi-trauma patients: correlation with patient severity. Cytokine. 2008;44(2):310-313.
37. Du W, Hu JK, Du W, Klein OD. Lineage tracing of epithelial cells in developing teeth reveals two strategies for building signaling centers. J Biol Chem. 2017;292(36):15062-15069.
38. Simon MP, Tournaire R, Pouyssegur J. The angiopoietin-2 gene of endothelial cells is up-regulated in hypoxia by a HIF binding site located in its first intron and by the central factors GATA-2 and Ets-1. J Cell Physiol. 2008;217(3):809-818.
39. Maddaluno L, Urwyler C, Werner S. Fibroblast growth factors: Key players in regeneration and tissue repair. Development 2017;144:4047-4060.
40. Kim R, Green JBA, Klein OD. From snapshots to movies: Understanding early tooth development in four dimensions. Dev Dyn. 2017;246(6):442-450.
| Files | ||
| Issue | Vol 8, No 3 (Summer 2021) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/jcr.v8i3.8838 | |
| Keywords | ||
| Tooth movement Root resorption Fibroblast growth factor | ||
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How to Cite
1.
Feizbakhsh M, Razavi SM, Hajhashemi V, Mortazavi M. Effects of FGF on tooth movement and root resorption. J Craniomaxillofac Res. 2022;8(3):122-128.
