Mir-148 and Mir-375 Expression in Oral Squamous Cell Carcinoma Patients with Oral Infection in Comparison with Oscc Patients without Oral Infection
-
Naghmeh Bahrami
,
Farzaneh Hosseini
,
Mahsa Hajmali
,
Abdolreza Mohamadnia
,
Mona Mohajeri Tehrani
,
Masoume Farhangiyan
Abstract
Introduction: Oral squamous cell carcinoma is a multifactorial disease that is the sixth most common cancer worldwide. MicroRNAs have been confirmed to play a role in oral squamous cell carcinoma, acting as either oncogenes or tumor suppressor genes. This study examined the expression level and role of microR-148 and microR-375 in oral cancer.Materials and Methods: In this study, we used 30 cancer samples with infection and 30 cancer samples without infection. To analyze the expression of microRNA 375 and microRNA 148, we used real-time PCR. First, we extracted total RNA from the samples. Then, we generated cDNA from it. Finally, the obtained cDNA was used in the real-time PCR technique.Results: In cancer patients with oral infection, there was an increase in microRNA-148 expression and a decrease in microRNA-375 compared to cancer patients without oral infection.Conclusion: The downregulation of microRNA-375 and upregulation of microRNA-148 can be utilized as diagnostic biomarkers and prognostic factors in oral cancer.Keywords: Oral cancer; OSCC; Realtime pcr; MiR-148; MiR-375.
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15. Wong T-S, Liu X-B, Wong BY-H, Ng RW-M, Yuen AP-W, Wei WI. Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clinical Cancer Research. 2008;14(9):2588-92.
16. Lin S-C, Liu C-J, Lin J-A, Chiang W-F, Hung P-S, Chang K-W. miR-24 up-regulation in oral carcinoma: positive association from clinical and in vitro analysis. Oral oncology. 2010;46(3):204-8.
17. نریمانی, آرمیتا, حسینی, فرزانه, بهرامی, نغمه, محمدنیا. بررسی میانگین بیان MicroRNA-155 (miR-155) و Carcinoembryonic Antigen Messenger RNA (CEA mRNA) در خون محیطی بیماران مبتلا به کارسینوم سلول سنگفرشی دهانی (OSCC). مجله دانشکده پزشکی اصفهان. 2019;36(510):1597-601.
2. Little J, Miller C, Rhodus N. Dental management of the medically compromised patient 6th ed. Missouri: Mosby. 2002:387-9.
3. Lodish HF. Molecular cell biology: Macmillan; 2008.
4. Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nature reviews Molecular cell biology. 2005;6(5):376-85.
5. Cai Y, Yu X, Hu S, Yu J. A brief review on the mechanisms of miRNA regulation. Genomics, proteomics & bioinformatics. 2009;7(4):147-54.
6. Nagy Z, Igaz P. Introduction to microRNAs: Biogenesis, action, relevance of tissue micrornas in disease pathogenesis, diagnosis and therapy—the concept of circulating microRNAs. Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance. 2015:3-30.
7. Singh SR, Rameshwar P. MicroRNA in Development and in the Progression of Cancer: Springer; 2014.
8. Chen Y, Song Y-X, Wang Z-N. The microRNA-148/152 family: multi-faceted players. Molecular cancer. 2013;12:1-8.
9. Friedrich M, Pracht K, Mashreghi MF, Jäck HM, Radbruch A, Seliger B. The role of the miR‐148/‐152 family in physiology and disease. European journal of immunology. 2017;47(12):2026-38.
10. Fujita Y, Kojima K, Ohhashi R, Hamada N, Nozawa Y, Kitamoto A, et al. MiR-148a attenuates paclitaxel resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression. Journal of biological chemistry. 2010;285(25):19076-84.
11. Liu X, Zhan Z, Xu L, Ma F, Li D, Guo Z, et al. MicroRNA-148/152 impair innate response and antigen presentation of TLR-triggered dendritic cells by targeting CaMKIIα. The Journal of Immunology. 2010;185(12):7244-51.
12. Patoulias D. Is miRNA-375 a promising biomarker for early detection and monitoring of patients with type 2 diabetes? Archives of Medical Science-Atherosclerotic Diseases. 2018;3(1):119-22.
13. Liu CJ, Lin SC, Yang CC, Cheng HW, Chang KW. Exploiting salivary miR‐31 as a clinical biomarker of oral squamous cell carcinoma. Head & neck. 2012;34(2):219-24.
14. Zheng Y, Cui L, Sun W, Zhou H, Yuan X, Huo M, et al. MicroRNA-21 is a new marker of circulating tumor cells in gastric cancer patients. Cancer biomarkers. 2012;10(2):71-7.
15. Wong T-S, Liu X-B, Wong BY-H, Ng RW-M, Yuen AP-W, Wei WI. Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clinical Cancer Research. 2008;14(9):2588-92.
16. Lin S-C, Liu C-J, Lin J-A, Chiang W-F, Hung P-S, Chang K-W. miR-24 up-regulation in oral carcinoma: positive association from clinical and in vitro analysis. Oral oncology. 2010;46(3):204-8.
17. نریمانی, آرمیتا, حسینی, فرزانه, بهرامی, نغمه, محمدنیا. بررسی میانگین بیان MicroRNA-155 (miR-155) و Carcinoembryonic Antigen Messenger RNA (CEA mRNA) در خون محیطی بیماران مبتلا به کارسینوم سلول سنگفرشی دهانی (OSCC). مجله دانشکده پزشکی اصفهان. 2019;36(510):1597-601.
| Files | ||
| Issue | Vol 11, No 1 (Winter 2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/jcr.v11i1.16477 | |
| Keywords | ||
| Oral cancer; OSCC; Realtime pcr; MiR-148; MiR-375. | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Bahrami N, Hosseini F, Hajmali M, Mohamadnia A, Mohajeri Tehrani M, Farhangiyan M. Mir-148 and Mir-375 Expression in Oral Squamous Cell Carcinoma Patients with Oral Infection in Comparison with Oscc Patients without Oral Infection. J Craniomaxillofac Res. 2024;11(1):20-25.
