Salivary antioxidant levels in pemphigus vulgaris patients compared to the healthy people
AbstractIntroduction: Pemphigus comprises a group of diseases characterized by cutaneous and mucosal blistering. Pemphigus vulgaris (PV) is the most common and important variant. Recent reports have revealed the relatively high incidence of PV in Iran. The onset or aggravation of many human diseases is attributed to oxidative stress and it has been suggested as one of the several factors responsible for etiopathogenesis of pemphigus. The present study sought to assess the association of salivary level of antioxidant enzyme glutathione peroxidase (GPx) and total antioxidant level with pemphigus vulgaris.Materials and Methods: This case-control study evaluated patients referred to Razi Dermatology Hospital in Tehran , Iran from January to November 2011. Thirty patients with early diagnosed PV, who had never been on treatment were enrolled in this study. The diagnosis of PV was confirmed by histopathology and direct immunofluorescence. The control group consisted of 30 age- and sex-matched healthy non-smoker volunteers.Saliva was collected from subjects for evaluation of glutathione peroxidase and total antioxidant levels and comparison with controls. GPX and TAS activity was analyzed by spectrophotometry . Independent samples t-test was applied to compare the means of continuous variables. Statistical analysis was performed using SPSS software. P ≤ 0.05 was considered significant.Results: The mean salivary level of glutathione peroxidase was significantly lower in pemphigus patients. No statistically significant difference was observed between the groups in total antioxidant levels of saliva.Conclusion: Our findings indicated that lower levels of glutathione peroxidase may be associated with pemphigus vulgaris. Salivary GPx level may be used for diagnosis of PV.Key words: Antioxidant, Pemphigus vulgaris, Glutathione peroxidase.
Veldman C, Feliciani C. Pemphigus. a complex T cell-dependent autoimmune disorder leading to acantholysis. Clin Rev Allergy Immunol. 2008 Jun; 34(3):313-20.
Scully C, Challacombe SJ. Pemphigus vulgaris. update on etiopathogenesis, oral manifestations, and management. Crit Rev Oral Biol Med. 2002;13(5):397-408.
Thorat MS, Raju A, Pradeep AR. Pemphigus vul- garis. effects on periodontal health. J Oral Sci.2010 Sep 52(3):449-54.
Chams-Davatchi C, Valikhani M, Daneshpazhooh M, Esmaili N, Balighi K ,Hallaji Z, et al. Pemphi- gus. analysis of 1209 cases. Int J Dermatol. 2005 Jun; 44(6):470-6.
Shirakata Y, Amagai M, Hanakawa Y, Nishikawa T, Hashimoto K. Lack of mucosal involvement in pemphigus foliaceus may be due to low expres- sion of desmoglein 1. J Invest Dermatol. 1998 Jan;110(1):76-8.
Asilian A, Yoosefi A, Faghini G. Pemphigus vul- garis in Iran: epidemiology and clinical profile. Skinmed. 2006 Mar-Apr; 5(2):69-71.
Naziroglu M, Kokcam I, Simsek H, Karakilcik AZ. Lipid peroxidation and antioxidants in plasma and red blood cells from patients with pemphi- gus vulgaris. J Basic Clin Physiol Pharmacol. 2003;14(1):31-42.
Battino M, Ferreiro MS, Gallardo I, Newman HN, Bullon P. The antioxidant capacity of saliva. J Clin Periodontol. 2002 Mar; 29(3):189-94.
Scully C, Gorsky M, Lozada-Nur F. The diagnosis and management of recurrent aphthous stomati- tis. a consensus approach. J Am Dent Assoc. 2003 Feb; 134(2):200-7.
Porter SR, Scully C, Pedersen A. Recurrent aph- thous stomatitis. Crit Rev Oral Biol Med. 1998;9(3):306-21.
Chambers JC, Haskard DO, Kooner JS. Vascular endothelial function and oxidative stress mecha- nisms in patients with Behcet’s syndrome. J Am Coll Cardiol. 2001 Feb; 37(2):517-20.
Beevi SS, Rasheed AM, Geetha A. Evaluation of oxidative stress and nitric oxide levels in patients with oral cavity cancer. Jpn J Clin Oncol. 2004 Jul;34(7):379-85.
Karincaoglu Y, Batcioglu K, Erdem T, Esrefoglu M, Genc M. The levels of plasma and salivary an- tioxidants in the patient with recurrent aphthous stomatitis. J Oral Pathol Med. 2005 Jan; 34(1):7- 12.
Cimen MY, Kaya TI, Eskandari G, Tursen U, Ikizoglu G, Atik U. Oxidant/antioxidant status in patients with recurrent aphthous stomatitis. Clin Exp Dermatol. 2003 Nov; 28(6):647-50.
Harman KE, Gratian MJ, Seed PT, Bhogal BS, Challacombe SJ, Black MM. Diagnosis of pemphi- gus by ELISA. a critical evaluation of two ELISAs for the detection of antibodies to the major pem- phigus antigens, desmoglein 1 and 3. Clin Exp Dermatol. 2000 May; 25(3):236-40.
Harman KE, Albert S, Black MM. Guidelines for the management of pemphigus vulgaris. Br J Der- matol. 2003 Nov; 149(5):926-37.
Ahmed AR, Spigelman Z, Cavacini LA, Posner MR. Treatment of pemphigus vulgaris with ritux- imab and intravenous immune globulin. N Engl J Med. 2006 Oct 26; 355(17):1772-9.
|Issue||Vol 3, No 1 (Winter 2016)|
|Antioxidant Pemphigus vulgaris Glutathione peroxidase|
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